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multiscribe reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe reverse transcriptase kit
    Multiscribe Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase/multiscribe+reverse+transcriptase+kit/pmc12260516-107-13-17
    Average 90 stars, based on 1 article reviews
    multiscribe reverse transcriptase kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Method and system for photoacoustic imaging of tissue and organ fibrosis
    Article Snippet: Statistical analysis was performed using Matlab 2018b (MathWorks, Inc., Natick, MA). .. RNA was then reverse transcribed into cDNA with Multiscribe Reverse Transcriptase (Thermo Fisher Scientific, Cat. #4311235), and levels of Col1a1, Col3a1, Col4a1, Acta2, and/or Gapdh were quantified. ..

    Article Title: Expression of a protein disulfide isomerase A3 variant associated with amyotrophic lateral sclerosis triggers disease features in mice.
    Article Snippet: Chemiluminescence signal and protein ladder images were acquired using the ChemiDoc Imaging System (BioRad). .. For RT-PCR, cDNA was synthesized from a total of 1 μg of RNA using Multiscribe Reverse Transcriptase (ThermoFisher Scientific) and random primers p(dN)6 (Roche) according to manufacturer’s instructions. .. Conventional RT-PCR was performed using GoTaq Green Master Mix (Promega).

    Article Title: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
    Article Snippet: .. We have included a description in the figure legend regarding how our RT-qPCR data is normalized and clarified it in the Methods section as follows (line 528): “The RNA was reverse transcribed using Multiscribe reverse transcriptase (ThermoFisher). .. TaqMan probes specific to the assessed miRNAs were used to measure miRNA levels, while SYBR Green master mix (Biorad) with target-specific oligonucleotides was employed to assess the expression levels of the miRNA targets.

    Article Title: Statins may enhance the chemosensitivity of R-CHOP in diffuse large B-cell lymphoma
    Article Snippet: [ 24 ] Briefly, total RNA was isolated with the Purelink RNA kit (Invitrogen) according to the manufactureŕs instructions. .. One microgram RNA was reverse transcribed by using the Multiscribe Reverse Transcriptase (Thermo Fischer) and the complementary DNA (cDNA) was used for a FAM-based real-time PCR. .. A standard protocol was used for the analysis of gene expressions (Applied Biosystems).

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat-Effects of Aging.
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 ◦C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat—Effects of Aging
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 °C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Article Title: A primary cell model of the very preterm epithelium reveals barrier defects at one year of age
    Article Snippet: RNA purity and yield was assessed using a Nanodrop 2000 (Thermo ScientificTM, Waltham, USA) before conversion to cDNA. .. Reverse transcription was carried out in 20 μL volumes using sample RNA, 1x RT buffer (Thermo ScientificTM, Waltham, USA), 5.5 mM MgCl2 (Thermo ScientificTM, Waltham, USA), 0.5 mM of each dNTP, random hexamers (InvitrogenTM, Waltham, USA), RNase inhibitor (InvitrogenTM, Waltham, USA), Multiscribe Reverse Transcriptase (InvitrogenTM, Waltham, USA) and RNase-DNase-free water (Thermo ScientificTM, Waltham, USA) using a BioRad CFX96 thermocycler (Bio-Rad, Hercules, USA) with standard reaction conditions of 25 °C for 10 minutes, 48 °C for 30 minutes, and 95 °C for 5 minutes. qPCR All qPCR reactions were performed using a QuantstudioTM 7 Flex (Applied BiosystemsTM, Waltham, USA) where samples were initially heated to 50 °C for 2 minutes and 95 °C for 10 minutes, followed by 40 PCR cycles of template denaturation at 95°C for 15 seconds and primer on June 17, 2025 by guest. ..

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    Synthesized:

    Article Title: Expression of a protein disulfide isomerase A3 variant associated with amyotrophic lateral sclerosis triggers disease features in mice.
    Article Snippet: Chemiluminescence signal and protein ladder images were acquired using the ChemiDoc Imaging System (BioRad). .. For RT-PCR, cDNA was synthesized from a total of 1 μg of RNA using Multiscribe Reverse Transcriptase (ThermoFisher Scientific) and random primers p(dN)6 (Roche) according to manufacturer’s instructions. .. Conventional RT-PCR was performed using GoTaq Green Master Mix (Promega).

    Quantitative RT-PCR:

    Article Title: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
    Article Snippet: .. We have included a description in the figure legend regarding how our RT-qPCR data is normalized and clarified it in the Methods section as follows (line 528): “The RNA was reverse transcribed using Multiscribe reverse transcriptase (ThermoFisher). .. TaqMan probes specific to the assessed miRNAs were used to measure miRNA levels, while SYBR Green master mix (Biorad) with target-specific oligonucleotides was employed to assess the expression levels of the miRNA targets.

    Real-time Polymerase Chain Reaction:

    Article Title: Statins may enhance the chemosensitivity of R-CHOP in diffuse large B-cell lymphoma
    Article Snippet: [ 24 ] Briefly, total RNA was isolated with the Purelink RNA kit (Invitrogen) according to the manufactureŕs instructions. .. One microgram RNA was reverse transcribed by using the Multiscribe Reverse Transcriptase (Thermo Fischer) and the complementary DNA (cDNA) was used for a FAM-based real-time PCR. .. A standard protocol was used for the analysis of gene expressions (Applied Biosystems).

    Article Title: A primary cell model of the very preterm epithelium reveals barrier defects at one year of age
    Article Snippet: RNA purity and yield was assessed using a Nanodrop 2000 (Thermo ScientificTM, Waltham, USA) before conversion to cDNA. .. Reverse transcription was carried out in 20 μL volumes using sample RNA, 1x RT buffer (Thermo ScientificTM, Waltham, USA), 5.5 mM MgCl2 (Thermo ScientificTM, Waltham, USA), 0.5 mM of each dNTP, random hexamers (InvitrogenTM, Waltham, USA), RNase inhibitor (InvitrogenTM, Waltham, USA), Multiscribe Reverse Transcriptase (InvitrogenTM, Waltham, USA) and RNase-DNase-free water (Thermo ScientificTM, Waltham, USA) using a BioRad CFX96 thermocycler (Bio-Rad, Hercules, USA) with standard reaction conditions of 25 °C for 10 minutes, 48 °C for 30 minutes, and 95 °C for 5 minutes. qPCR All qPCR reactions were performed using a QuantstudioTM 7 Flex (Applied BiosystemsTM, Waltham, USA) where samples were initially heated to 50 °C for 2 minutes and 95 °C for 10 minutes, followed by 40 PCR cycles of template denaturation at 95°C for 15 seconds and primer on June 17, 2025 by guest. ..

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    cDNA Synthesis:

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat-Effects of Aging.
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 ◦C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat—Effects of Aging
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 °C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Polymerase Chain Reaction:

    Article Title: A primary cell model of the very preterm epithelium reveals barrier defects at one year of age
    Article Snippet: RNA purity and yield was assessed using a Nanodrop 2000 (Thermo ScientificTM, Waltham, USA) before conversion to cDNA. .. Reverse transcription was carried out in 20 μL volumes using sample RNA, 1x RT buffer (Thermo ScientificTM, Waltham, USA), 5.5 mM MgCl2 (Thermo ScientificTM, Waltham, USA), 0.5 mM of each dNTP, random hexamers (InvitrogenTM, Waltham, USA), RNase inhibitor (InvitrogenTM, Waltham, USA), Multiscribe Reverse Transcriptase (InvitrogenTM, Waltham, USA) and RNase-DNase-free water (Thermo ScientificTM, Waltham, USA) using a BioRad CFX96 thermocycler (Bio-Rad, Hercules, USA) with standard reaction conditions of 25 °C for 10 minutes, 48 °C for 30 minutes, and 95 °C for 5 minutes. qPCR All qPCR reactions were performed using a QuantstudioTM 7 Flex (Applied BiosystemsTM, Waltham, USA) where samples were initially heated to 50 °C for 2 minutes and 95 °C for 10 minutes, followed by 40 PCR cycles of template denaturation at 95°C for 15 seconds and primer on June 17, 2025 by guest. ..

    Quantitation Assay:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    Random Hexamer:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    Over Expression:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..



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